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Epitope-directed selection of monoclonal antibodies specific for plant cell walls: isolation of plant cell-wall-specific antibodies for ELISA.
Screening of a phage display antibody library using immobilized plant cell wall polysaccharides has been applied to the isolation of plant cell-wall-specific antibodies for enzyme-linked immunosorbent assays (ELISA). Phage displaying antibody fragments from the library, with binding specificity for arabinoxylan (AX), were detected by biotinylated analogues of these polysaccharides. Affinity purification of selected phages on immobilized AX-specific monoclonal antibodies resulted in antibody-producing phage particles be359ba680
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